nadph-glotm assay kit Search Results


nadp + /nadph-glotm kit #g9081  (Promega)
90
Promega nadp + /nadph-glotm kit #g9081
(A) Respiratory profile for non-treated control DRG neurons (black) shows baseline OCR of ~180 pmoles O2/min. Sequential, in port additions of mitochondrial effectors (vertical arrows), reveal that ~90% of total oxygen consumption feed mitochondrial respiration: of this OCR ~70% support ATP synthesis and ~30% is lost to proton leak. Spare respiratory capacity (SRC), i.e., FCCP-induced increase in OCR over the baseline is ~250% for control DRGs. Following ara-C exposure baseline OCR (red) drops by ~30% and maximal respiration by nearly 50% (FCCP-induced), with ~30% reduction in SRC and ~ 20% increase in OCR feeding the proton leak. (Bottom) Oxygen tension in control wells is reduced by the addition of FCCP and even further by 2-deoxyglucose (2DG). Oxygen tension is reduced to a lesser extent in ara-C exposed DRGs, reflecting diminished mitochondrial OCR. (B) Each parameter calculated for control cultures was assigned the value of 100%; effects of treatment were calculated as percent change relative to each respective control. Data are presented as mean±SEM of four independent biological experiments. (C) NADPH levels are decreased and <t>NADP+/NADPH</t> ratios increased by ara-C. Concomitant addition of 6AN increased these effects. Data are mean±SEM for 3 independent experiments; * different from control; **different from ara-C; P< 0.05. (D) GSH levels were reduced by ara-C and further depleted by 6AN. Data are given as mean±SEM calculated from 3 independent experiments; * different from control; **different from ara-C; P< 0.05
Nadp + /Nadph Glotm Kit #G9081, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph-glotm+assay+kit/nad+nadh+glo+assay+kit/pmc05971160-104-16-21
Average 90 stars, based on 1 article reviews
nadp + /nadph-glotm kit #g9081 - by Bioz Stars, 2026-09
90/100 stars
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nadp/nadph-glotm assay  (Promega)
90
Promega nadp/nadph-glotm assay
(A) Respiratory profile for non-treated control DRG neurons (black) shows baseline OCR of ~180 pmoles O2/min. Sequential, in port additions of mitochondrial effectors (vertical arrows), reveal that ~90% of total oxygen consumption feed mitochondrial respiration: of this OCR ~70% support ATP synthesis and ~30% is lost to proton leak. Spare respiratory capacity (SRC), i.e., FCCP-induced increase in OCR over the baseline is ~250% for control DRGs. Following ara-C exposure baseline OCR (red) drops by ~30% and maximal respiration by nearly 50% (FCCP-induced), with ~30% reduction in SRC and ~ 20% increase in OCR feeding the proton leak. (Bottom) Oxygen tension in control wells is reduced by the addition of FCCP and even further by 2-deoxyglucose (2DG). Oxygen tension is reduced to a lesser extent in ara-C exposed DRGs, reflecting diminished mitochondrial OCR. (B) Each parameter calculated for control cultures was assigned the value of 100%; effects of treatment were calculated as percent change relative to each respective control. Data are presented as mean±SEM of four independent biological experiments. (C) NADPH levels are decreased and <t>NADP+/NADPH</t> ratios increased by ara-C. Concomitant addition of 6AN increased these effects. Data are mean±SEM for 3 independent experiments; * different from control; **different from ara-C; P< 0.05. (D) GSH levels were reduced by ara-C and further depleted by 6AN. Data are given as mean±SEM calculated from 3 independent experiments; * different from control; **different from ara-C; P< 0.05
Nadp/Nadph Glotm Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph-glotm+assay+kit/gsh+gssg+glo+assay/pmc10891823-103-14-11
Average 90 stars, based on 1 article reviews
nadp/nadph-glotm assay - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
nadp/nadph-glotm kit  (Promega)
90
Promega nadp/nadph-glotm kit
(A) Respiratory profile for non-treated control DRG neurons (black) shows baseline OCR of ~180 pmoles O2/min. Sequential, in port additions of mitochondrial effectors (vertical arrows), reveal that ~90% of total oxygen consumption feed mitochondrial respiration: of this OCR ~70% support ATP synthesis and ~30% is lost to proton leak. Spare respiratory capacity (SRC), i.e., FCCP-induced increase in OCR over the baseline is ~250% for control DRGs. Following ara-C exposure baseline OCR (red) drops by ~30% and maximal respiration by nearly 50% (FCCP-induced), with ~30% reduction in SRC and ~ 20% increase in OCR feeding the proton leak. (Bottom) Oxygen tension in control wells is reduced by the addition of FCCP and even further by 2-deoxyglucose (2DG). Oxygen tension is reduced to a lesser extent in ara-C exposed DRGs, reflecting diminished mitochondrial OCR. (B) Each parameter calculated for control cultures was assigned the value of 100%; effects of treatment were calculated as percent change relative to each respective control. Data are presented as mean±SEM of four independent biological experiments. (C) NADPH levels are decreased and <t>NADP+/NADPH</t> ratios increased by ara-C. Concomitant addition of 6AN increased these effects. Data are mean±SEM for 3 independent experiments; * different from control; **different from ara-C; P< 0.05. (D) GSH levels were reduced by ara-C and further depleted by 6AN. Data are given as mean±SEM calculated from 3 independent experiments; * different from control; **different from ara-C; P< 0.05
Nadp/Nadph Glotm Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph-glotm+assay+kit/mao+glotm+assay+kit/pmc06478810-57-9-11
Average 90 stars, based on 1 article reviews
nadp/nadph-glotm kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
nadp/nadphglotm assay  (Promega)
90
Promega nadp/nadphglotm assay
(A) Respiratory profile for non-treated control DRG neurons (black) shows baseline OCR of ~180 pmoles O2/min. Sequential, in port additions of mitochondrial effectors (vertical arrows), reveal that ~90% of total oxygen consumption feed mitochondrial respiration: of this OCR ~70% support ATP synthesis and ~30% is lost to proton leak. Spare respiratory capacity (SRC), i.e., FCCP-induced increase in OCR over the baseline is ~250% for control DRGs. Following ara-C exposure baseline OCR (red) drops by ~30% and maximal respiration by nearly 50% (FCCP-induced), with ~30% reduction in SRC and ~ 20% increase in OCR feeding the proton leak. (Bottom) Oxygen tension in control wells is reduced by the addition of FCCP and even further by 2-deoxyglucose (2DG). Oxygen tension is reduced to a lesser extent in ara-C exposed DRGs, reflecting diminished mitochondrial OCR. (B) Each parameter calculated for control cultures was assigned the value of 100%; effects of treatment were calculated as percent change relative to each respective control. Data are presented as mean±SEM of four independent biological experiments. (C) NADPH levels are decreased and <t>NADP+/NADPH</t> ratios increased by ara-C. Concomitant addition of 6AN increased these effects. Data are mean±SEM for 3 independent experiments; * different from control; **different from ara-C; P< 0.05. (D) GSH levels were reduced by ara-C and further depleted by 6AN. Data are given as mean±SEM calculated from 3 independent experiments; * different from control; **different from ara-C; P< 0.05
Nadp/Nadphglotm Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph-glotm+assay+kit/assay+kit/pmc05031559__mmc2-482-12-16
Average 90 stars, based on 1 article reviews
nadp/nadphglotm assay - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
nad + /nadh-glotm  (Promega)
90
Promega nad + /nadh-glotm
(A) Respiratory profile for non-treated control DRG neurons (black) shows baseline OCR of ~180 pmoles O2/min. Sequential, in port additions of mitochondrial effectors (vertical arrows), reveal that ~90% of total oxygen consumption feed mitochondrial respiration: of this OCR ~70% support ATP synthesis and ~30% is lost to proton leak. Spare respiratory capacity (SRC), i.e., FCCP-induced increase in OCR over the baseline is ~250% for control DRGs. Following ara-C exposure baseline OCR (red) drops by ~30% and maximal respiration by nearly 50% (FCCP-induced), with ~30% reduction in SRC and ~ 20% increase in OCR feeding the proton leak. (Bottom) Oxygen tension in control wells is reduced by the addition of FCCP and even further by 2-deoxyglucose (2DG). Oxygen tension is reduced to a lesser extent in ara-C exposed DRGs, reflecting diminished mitochondrial OCR. (B) Each parameter calculated for control cultures was assigned the value of 100%; effects of treatment were calculated as percent change relative to each respective control. Data are presented as mean±SEM of four independent biological experiments. (C) NADPH levels are decreased and <t>NADP+/NADPH</t> ratios increased by ara-C. Concomitant addition of 6AN increased these effects. Data are mean±SEM for 3 independent experiments; * different from control; **different from ara-C; P< 0.05. (D) GSH levels were reduced by ara-C and further depleted by 6AN. Data are given as mean±SEM calculated from 3 independent experiments; * different from control; **different from ara-C; P< 0.05
Nad + /Nadh Glotm, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph-glotm+assay+kit/nad+p+h+glotm+detection+system/pmc06039359-107-9-24
Average 90 stars, based on 1 article reviews
nad + /nadh-glotm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
bactiter-glotm microbial cell viability assay kit  (Promega)
90
Promega bactiter-glotm microbial cell viability assay kit
(A) Respiratory profile for non-treated control DRG neurons (black) shows baseline OCR of ~180 pmoles O2/min. Sequential, in port additions of mitochondrial effectors (vertical arrows), reveal that ~90% of total oxygen consumption feed mitochondrial respiration: of this OCR ~70% support ATP synthesis and ~30% is lost to proton leak. Spare respiratory capacity (SRC), i.e., FCCP-induced increase in OCR over the baseline is ~250% for control DRGs. Following ara-C exposure baseline OCR (red) drops by ~30% and maximal respiration by nearly 50% (FCCP-induced), with ~30% reduction in SRC and ~ 20% increase in OCR feeding the proton leak. (Bottom) Oxygen tension in control wells is reduced by the addition of FCCP and even further by 2-deoxyglucose (2DG). Oxygen tension is reduced to a lesser extent in ara-C exposed DRGs, reflecting diminished mitochondrial OCR. (B) Each parameter calculated for control cultures was assigned the value of 100%; effects of treatment were calculated as percent change relative to each respective control. Data are presented as mean±SEM of four independent biological experiments. (C) NADPH levels are decreased and <t>NADP+/NADPH</t> ratios increased by ara-C. Concomitant addition of 6AN increased these effects. Data are mean±SEM for 3 independent experiments; * different from control; **different from ara-C; P< 0.05. (D) GSH levels were reduced by ara-C and further depleted by 6AN. Data are given as mean±SEM calculated from 3 independent experiments; * different from control; **different from ara-C; P< 0.05
Bactiter Glotm Microbial Cell Viability Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph-glotm+assay+kit/bactiter+glo+microbial+cell+viability+assay/pm30805924-63-5-10
Average 90 stars, based on 1 article reviews
bactiter-glotm microbial cell viability assay kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


(A) Respiratory profile for non-treated control DRG neurons (black) shows baseline OCR of ~180 pmoles O2/min. Sequential, in port additions of mitochondrial effectors (vertical arrows), reveal that ~90% of total oxygen consumption feed mitochondrial respiration: of this OCR ~70% support ATP synthesis and ~30% is lost to proton leak. Spare respiratory capacity (SRC), i.e., FCCP-induced increase in OCR over the baseline is ~250% for control DRGs. Following ara-C exposure baseline OCR (red) drops by ~30% and maximal respiration by nearly 50% (FCCP-induced), with ~30% reduction in SRC and ~ 20% increase in OCR feeding the proton leak. (Bottom) Oxygen tension in control wells is reduced by the addition of FCCP and even further by 2-deoxyglucose (2DG). Oxygen tension is reduced to a lesser extent in ara-C exposed DRGs, reflecting diminished mitochondrial OCR. (B) Each parameter calculated for control cultures was assigned the value of 100%; effects of treatment were calculated as percent change relative to each respective control. Data are presented as mean±SEM of four independent biological experiments. (C) NADPH levels are decreased and NADP+/NADPH ratios increased by ara-C. Concomitant addition of 6AN increased these effects. Data are mean±SEM for 3 independent experiments; * different from control; **different from ara-C; P< 0.05. (D) GSH levels were reduced by ara-C and further depleted by 6AN. Data are given as mean±SEM calculated from 3 independent experiments; * different from control; **different from ara-C; P< 0.05

Journal: Free radical biology & medicine

Article Title: Neurotoxicity of Cytarabine (Ara-C) in Dorsal Root Ganglion Neurons Originates from Impediment of mtDNA Synthesis and Compromise of Mitochondrial Function

doi: 10.1016/j.freeradbiomed.2018.04.570

Figure Lengend Snippet: (A) Respiratory profile for non-treated control DRG neurons (black) shows baseline OCR of ~180 pmoles O2/min. Sequential, in port additions of mitochondrial effectors (vertical arrows), reveal that ~90% of total oxygen consumption feed mitochondrial respiration: of this OCR ~70% support ATP synthesis and ~30% is lost to proton leak. Spare respiratory capacity (SRC), i.e., FCCP-induced increase in OCR over the baseline is ~250% for control DRGs. Following ara-C exposure baseline OCR (red) drops by ~30% and maximal respiration by nearly 50% (FCCP-induced), with ~30% reduction in SRC and ~ 20% increase in OCR feeding the proton leak. (Bottom) Oxygen tension in control wells is reduced by the addition of FCCP and even further by 2-deoxyglucose (2DG). Oxygen tension is reduced to a lesser extent in ara-C exposed DRGs, reflecting diminished mitochondrial OCR. (B) Each parameter calculated for control cultures was assigned the value of 100%; effects of treatment were calculated as percent change relative to each respective control. Data are presented as mean±SEM of four independent biological experiments. (C) NADPH levels are decreased and NADP+/NADPH ratios increased by ara-C. Concomitant addition of 6AN increased these effects. Data are mean±SEM for 3 independent experiments; * different from control; **different from ara-C; P< 0.05. (D) GSH levels were reduced by ara-C and further depleted by 6AN. Data are given as mean±SEM calculated from 3 independent experiments; * different from control; **different from ara-C; P< 0.05

Article Snippet: Measurement of NADP + /NADPH and GSH/GSSG ratios Intracellular NADP + /NADPH ratios were measured using NADP + /NADPH-GloTM kit (#G9081, Promega) according to the manufacturer.

Techniques: Control